Human / Cynomolgus / Rhesus macaque CD28 Protein, Fc Tag, active dimer (MALS verified)

Human / Cynomolgus / Rhesus macaque CD28, Fc Tag (CD8-H525a) is expressed from human 293 cells (HEK293) . It contains AA Asn 19 - Pro 152 (Accession # P10747-1) . In the region Asn 19 - Pro 152, the AA sequence of Human, Cynomolgus and Rhesus macaque CD28 are homologus.

Product Specifications

Background

T-cell-specific surface glycoprotein CD28 is also known as TP44, is a single-pass type I membrane protein which contains one Ig-like V-type (immunoglobulin-like) domain. is one of the molecules expressed on T cells that provide co-stimulatory signals, which are required for T cell activation. CD28 is the receptor for CD80 (B7.1) and CD86 (B7.2) . When activated by Toll-like receptor ligands, the CD80 expression is upregulated in antigen presenting cells (APCs) . The CD86 expression on antigen presenting cells is constitutive. CD28 is the only B7 receptor constitutively expressed on naive T cells.

Specifications

This protein carries a human IgG1 Fc tag at the C-terminus. The protein has a calculated MW of 41.6 kDa. The protein migrates as 53-66 kDa under reducing (R) condition, and 100-120 kDa under non-reducing (NR) condition (SDS-PAGE) due to glycosylation.

Accession Number

P10747-1

Expression Region

Asn 19 - Pro 152

Host

HEK293

Target

CD28

Conjugation

Unconjugated

Tag

C-Fc

Source

Human / Cynomolgus / Rhesus macaque

Stability

-20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Endotoxin

1.0 EU per μg

Purity

95%

Bioactivity

Immobilized Human / Cynomolgus / Rhesus macaque CD28, Fc Tag (Cat. No. CD8-H525a) at 2 μg/mL (100 μL/well) can bind Anti-Mouse CD28 MAb with a linear range of 0.1-2 ng/mL (QC tested).