Canine CD3 epsilon & CD3 delta Heterodimer Protein, His Tag&Tag Free (MALS verified)

T-cell surface glycoprotein CD3 delta & CD3 epsilon chain, also known as CD3D & CD3E or CD3D&CD3E respectively, are single-pass type I membrane proteins. CD3D, together with CD3- epsilon (CD3E), CD3-gamma and CD3-zeta, and the T-cell receptor alpha/beta and gamma/delta heterodimers, forms the T cell receptor-CD3 complex. T cell receptor-CD3 complex plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways.

Product Specifications

Background

Specifications

Canine CD3E & CD3D Heterodimer Protein, His Tag&Tag Free is produced by co-expression of CD3E and CD3D, has a calculated MW of 16.6 kDa (CD3E) and 13.3 kDa (CD3D) . Subunit CD3E is fused with a polyhistidine tag at the C-terminus and subunit CD3D contains no tag. As a result of glycosylation, the heterodimer protein migrates as 19-20 kDa (CD3E) under reducing (R) condition, and 36-45 kDa (CD3D) under non-reducing (NR) condition (SDS-PAGE) .

Accession Number

P27597-1 (CD3E) & XP_536556.1 (CD3D)

Expression Region

Gln 22 - Leu 122 (CD3E) & Phe 22 - Thr 103 (CD3D)

Host

HEK293

Target

CD3E & CD3D

Conjugation

Unconjugated

Tag

C-10xHis | Native

Source

Canine

Stability

-20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower.