Human FcRn binding Kit (TR-FRET)

FCGRT & B2M heterodimer protein (FcRn complex) consist of two subunits: p51 (equivalent to FCGRT), and p14 (equivalent to beta-2-microglobulin), and forms an MHC class I-like heterodimer. Fc fragment of IgG, receptor, transporter, alpha (FCGRT) binds to the Fc region of monomeric immunoglobulins gamma and mediates the uptake of IgG from milk. FCGRT possible role in transfer of immunoglobulin G from mother to fetus. Beta-2-microglobulin (B2M) is a component of the class I major histocompatibility complex (MHC) and involved in the presentation of peptide antigens to the immune system.

This kit is designed to facilitate the half-life evaluation of antibody drug candidates, and also high-throughput screening of FcRn inhibitors. It can also be used as a universal detection tool to identifying the ability of antibody drugs to bind to FcRn.

1. Store the unopened kit at 2-8°C upon receipt. 2. Locate the expiration date on the outer packaging and do not use reagents beyond their expiration date. 3. The opened kit should be stored according to the storage conditions listed in the Components Table. The shelf life of the opened kit is 30 days from the date of opening.

This Human FcRn binding Kit (TR-FRET) is based on TR-FRET technology (Time-Resolved Fluorescence Resonance Energy Transfer) . Use the mixture of biotinylated FcRn and Europium-chelate labeled streptavidin as the donor, FA labeled Human IgG1 antibody as the acceptor. - In the absence of FcRn-binding components, the donor and acceptor are in close proximity due to the binding of FcRn and FA-labeled Human IgG1 antibody. The donor emits a 620 nm signal upon excitation, which is absorbed by the acceptor, resulting in a 665 nm emission. - In the presence of FcRn-binding components, they interfere with the donor-acceptor interaction, preventing FRET from occurring.