Recombinant Human RSPO2

Product Specifications

Product Name Alternative

R-spondin-2, RSPO2, Roof plate-specific spondin-2, hRspo2, PRO34209

UniProt

Q6UXX9

Expression Region

22-205

Host

Mammalian Cells

Tag

C-terminal Flag Tag

Applications

Western Blot, ELISA

Field of Research

Neuroscience, Cardiovascular

Endotoxin

< 0.01 EU/µg as determined by LAL test.

Purity

Greater than 95% as determined by SDS-PAGE.

Activity

Measured by its ability to induce Topflash reporter activity in 293 human embryonic kidney cells. The typical ED50 is 86 ng/mL in the presence of 3.24 ng/mL Recombinant Human Wnt3a.

Form

Lyophilized powder

Reconstitution

Centrifuge the vial at 10000 rpm for 30 s before opening, reconstitute in sterile distilled water to a concentration of 0.1-1 mg/ml by gently pipetting 2-3 times, don't vortex.

Molecular Weight

22-28 kDa

Shipping Conditions

4°C with ice bag

Storage Conditions

The lyophilized protein is stable at -20 °C for up to 1 year. For extended storage, it is recommended to further dilute in working aliquots after reconstitution. The protein solution is stable at ≤ -20 °C for 3 months, or 2-7 days at 2-8 °C under sterile conditions.Avoid repeated freeze/thaw cycle.