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Anti-Bcl-2 (Phospho-Thr56) Antibody

Rabbit Polyclonal Antibody specific to Bcl-2 (Phospho-Thr56)

Product Specifications

Product Name Alternative

Anti-Bcl-2 (Phospho-Thr56) Rabbit Polyclonal Antibody

Gene Name

BCL2

Gene ID

151430

NCBI Gene ID

<a href="https://www.ncbi.nlm.nih.gov/gene/?term=BCL2">BCL2</a>

UniProt

P10415

Cellular Locus

Mitochondrion outer membrane, Nucleus membrane, Endoplasmic reticulum membrane, Cytoplasm

Host

Rabbit

Reactivity

Human

Immunogen

Peptide sequence that includes phosphorylation site of threonine 56 (G-H-T(p)-P-H) derived from human Bcl-2 and conjugated to KLH.

Target Antigen

Apoptosis regulator Bcl-2

Target

Bcl-2 (Phospho-Thr56)

Clonality

Polyclonal

Type

Antibody

Applications

IHC

Field of Research

Phosphospecific Antibodies

Purification Method

Affinity-purified on phosphopeptide; non-phosphopeptidereactive antibodies were removed by chromatography on non-phosphorylated peptide

Concentration

1.0 mg/mL

Dilution

Dilute in PBS or medium that is identical to that used in the assay system.

Format

Purified

Form

Liquid

Buffer

Phosphate Buffered Saline

Function

Suppresses apoptosis in a variety of cell systems including factor-dependent lymphohematopoietic and neural cells (PubMed:1508712, PubMed:8183370). Regulates cell death by controlling the mitochondrial membrane permeability (PubMed:11368354). Appears to function in a feedback loop system with caspases (PubMed:11368354). Inhibits caspase activity either by preventing the release of cytochrome c from the mitochondria and/or by binding to the apoptosis-activating factor (APAF-1) (PubMed:11368354). Also acts as an inhibitor of autophagy: interacts with BECN1 and AMBRA1 during non-starvation conditions and inhibits their autophagy function (PubMed:18570871, PubMed:21358617, PubMed:20889974). May attenuate inflammation by impairing NLRP1-inflammasome activation, hence CASP1 activation and IL1B release (PubMed:17418785). {PubMed:1508712, PubMed:17418785, PubMed:18570871, PubMed:20889974, PubMed:21358617, PubMed:8183370, PubMed:11368354}.

Additionnal Information

<br><br>Immunohistochemistry: use at dilution of 1:50-1:100. <br><br>These are recommended working dilutions. <br><br>End user should determine optimal dilutions for their application.

Storage Conditions

This antibody is stable for at least one (1) year at -20°C.

Product Datasheet

https://www.qedbio.com/wp-content/uploads/pdf/43064.pdf

Specificity

This antibody detects endogenous human Bcl-2 only when phosphorylated at threonine 56.Accession no.: P10415, NP_000624.2

Formulation

PBS (without Mg2 and Ca2 ), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Buffer pH

pH 7.4

Target Background

Bcl-2 protein suppresses apoptosis in a variety of cellular systems including factor-dependent lymphohematopoietic and neural cells. It regulates cell death by controlling the mitochondrial membrane permeability and appears to function in a feedback loop system with caspase. Bcl-2 inhibits caspase acativity either by preventing release of cytochrome c from the mitochondria and/or by binding to apoptosis-activating factor (Apaf-1). The predicted molecular weight of Bcl-2 is ~26kDa.

CAS Number

9007-83-4

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