IPTG Solution 100 mM (Biotechnology Grade)

<strong>100 mM IPTG Solution</strong>_x000D_ <strong>Catalog number:</strong> B2010137_x000D_ <strong>Lot number:</strong> Batch Dependent_x000D_ <strong>Expiration Date:</strong> Batch dependent_x000D_ <strong>Volume/Weight:</strong> 10 x 1 mL_x000D_ <strong>pH:</strong> 7_x000D_ <strong>Supplied as:</strong> Ready-to-use solution_x000D_ <strong>Appearance:</strong> Clear solution_x000D_ <strong>Applications:</strong> optimized stock solution of IPTG (isopropyl-beta-D-thiogalactopyranoside), a synthetic analog of lactose. IPTG inactivates the lac repressor of the lac operon, trigeering the induction of beta-galactosidase synthesis. IPTG is used with X-Gal in the blue/white colony screening. it also used to induce the expression of recombinant protein under the control of the lac operon._x000D_ <strong>Storage:</strong> ""-20°C""_x000D_ <strong>Keywords:</strong> IPTG solution, IPTG inducer, IPTG expression, IPTG X-Gal_x000D_ <strong>Grade:</strong> Biotechnology grade. All components are highly pure (minimum 99%). All solutions are made with Type I ultrapure water (resistivity &gt;18 MΩ-cm) and are filtered on a 0.22 um._x000D_ _x000D_ <strong>References:</strong>_x000D_ 1: Tran DTM, Phan TTP, Huynh TK, Dang NTK, Huynh PTK, Nguyen TM, Truong TTT,_x000D_ Tran TL, Schumann W, Nguyen HD. Development of inducer-free expression plasmids_x000D_ based on IPTG-inducible promoters for Bacillus subtilis. Microb Cell Fact. 2017_x000D_ Jul 25;16(1):130._x000D_ 2: Marbach A, Bettenbrock K. lac operon induction in Escherichia coli:_x000D_ Systematic comparison of IPTG and TMG induction and influence of the_x000D_ transacetylase LacA. J Biotechnol. 2012 Jan;157(1):82-8._x000D_ _x000D_ 3: Wandrey G, Bier C, Binder D, Hoffmann K, Jaeger KE, Pietruszka J, Drepper T,_x000D_ Büchs J. Light-induced gene expression with photocaged IPTG for induction_x000D_ profiling in a high-throughput screening system. Microb Cell Fact. 2016 Apr_x000D_ 23;15:63._x000D_ 4: Kjaerulff S, Nielsen O. An IPTG-inducible derivative of the fission yeast nmt_x000D_ promoter. Yeast. 2015 Jun;32(6):469-78._x000D_ _x000D_ 5: Dvorak P, Chrast L, Nikel PI, Fedr R, Soucek K, Sedlackova M, Chaloupkova R,_x000D_ de Lorenzo V, Prokop Z, Damborsky J. Exacerbation of substrate toxicity by IPTG_x000D_ in Escherichia coli BL21(DE3) carrying a synthetic metabolic pathway. Microb_x000D_ Cell Fact. 2015 Dec 21;14:201._x000D_ 6: Zhang Z, Kuipers G, Niemiec Ł, Baumgarten T, Slotboom DJ, de Gier JW, Hjelm_x000D_ A. High-level production of membrane proteins in E. coli BL21(DE3) by omitting_x000D_ the inducer IPTG. Microb Cell Fact. 2015 Sep 16;14:142._x000D_ _x000D_ 7: Goodson KA, Wang Z, Haeusler AR, Kahn JD, English DS. LacI-DNA-IPTG loops:_x000D_ equilibria among conformations by single-molecule FRET. J Phys Chem B. 2013 Apr_x000D_ 25;117(16):4713-22._x000D_ 8: Ravishankar S, Ambady A, Ramu H, Mudugal NV, Tunduguru R, Anbarasu A, Sharma_x000D_ UK, Sambandamurthy VK, Ramaiah S. An IPTG Inducible Conditional Expression_x000D_ System for Mycobacteria. <a href="https://pubmed.ncbi.nlm.nih.gov/26247874/">PLoS One</a>. 2015 Aug 6;10(8):e0134562._x000D_ _x000D_ <strong>Products Related to 100mM IPTG Solution </strong> <a>Biochemical Buffers and Solutions</a>