Anti-Progesterone Receptor Antibody

Mouse Monoclonal Antibody specific to Progesterone Receptor

Product Specifications

CAS Number

9007-83-4

Product Name Alternative

PR, Nuclear receptor subfamily 3 group C member 3

Gene Name

PGR

Gene ID

5241

NCBI Gene ID

UniProt

P06401

Accession Number

NP_000917

Cellular Locus

Nucleus. Cytoplasm. Note=Nucleoplasmic shuttling is both hormone- and cell cycle-dependent. On hormone stimulation, retained in the cytoplasm in the G(1) and G(2)/M phases.; [Isoform A]: Nucleus. Cytoplasm. Note=Mainly nuclear.; [Isoform 4]: Mitochondrion outer membrane

Host

Mouse

Reactivity

Human

Immunogen

6-His fusion protein containing the region encoding aa 1-101 of human progesterone receptor expressed in E. coli.

Target Antigen

Progesterone receptor

Target

Progesterone Receptor

Clonality

Monoclonal

Isotype

IgG2a

Type

Antibody

Applications

Field of Research

Cancer Research

Purification Method

Purified by Protein G affinity chromatography

Concentration

Lot Specific

Dilution

Dilute in PBS or medium which is identical to that used in the assay system.

Format

Purified

Form

Liquid

Buffer

Phosphate Buffered Saline

Function

The steroid hormones and their receptors are involved in the regulation of eukaryotic gene expression and affect cellular proliferation and differentiation in target tissues. Depending on the isoform, progesterone receptor functions as transcriptional activator or repressor. {PubMed:10757795, PubMed:1587864, PubMed:9407067, PubMed:7969170, PubMed:8180103, PubMed:8264658, PubMed:10757795}.; [Isoform B]: Transcriptional activator of several progesteron-dependent promoters in a variety of cell types. Involved in activation of SRC-dependent MAPK signaling on hormone stimulation. {PubMed:7969170}.; [Isoform 4]: Increases mitochondrial membrane potential and cellular respiration upon stimulation by progesterone.

Additionnal Information

Immunoblotting: use at 1-5 ug/ml. <br><br>Positive controls: MCF7 cells or recombinant protein.

Storage Conditions

This antibody is stable for at least one (1) year at -70°C. Avoid multiple freeze-thaw cycles.

Specificity

This antibody recognizes human progesterone receptor (PgR), a member of the steroid family of nuclear receptors. PgR is found as a 94 kD protein (Form A) or a 120 kD protein (Form B). Binding of progesterone to its receptor results in a conformational change that allows dimerization and binding of the receptor to progesterone response elements and activated transcription. When bound to DNA, PgR can positively or negatively regulate gene transcription.