CheKine™ Micro Glucoamylase Activity Assay Kit

Glycosylase (EC3.2.1.3) also known as γ-amylase, which is an extracellular enzyme that is secreted by a series of microorganisms and has exonuclease activity. The main role of Glycosylase is to hydrolyze α-1,4 glycosidic bonds in sequence from non-reducing ends on the carbon chains such as starch, dextrin, glycogen, etc., and cut off each glucose unit. For amylopectin, when it encounters a branch point, it can also hydrolyze α-1,6 glycosidic bonds, thereby hydrolyzing all amylopectin to glucose. It is one of the important industrial enzyme preparations in China, and is widely used in alcohol, liquor, antibiotics, amino acids, organic acids, glycerol, starch sugar and other industries. CheKine TM Micro Glycosylase Activity Assay Kit can be used to detect biological samples such as animal and plant tissues, bacteria or cells, serum or plasma. In the kit, glycosylase converts soluble starch to glucose. Under the alkaline condition, 3.5-Dinitrosalicylic acid is reduced to brown-red amino compound by co-heating with glucose. The brown-red amino compound has a maximum absorption peak at 540 nm and the absorbance ratio is in direct proportion to the contents of glucose. In this kit, the glycosylase activity is quantified by measuring the color development at 540 nm.

The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Storage at ４°C and Keep from light immediately upon receipt. Kit has a storage time of 6 months from receipt. Refer to list of materials supplied for storage conditions of individual components.

• If not assayed immediately, samples can be stored at -80°C for one month.• It is recommended to perform several dilutions of your sample to ensure the readings are within the standard value range.• Fresh samples are necessary for good results. If don't perform the assay at the same time, it's better to complete the Sample Preparation step before storing the samples.