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Biotinylated Prostate Specific Antigen Antibody / PSA

This antibody reacts to native human PSA (Prostate Specific Antigen), including both free (unbound) form and in a complex with ACT (alpha 1-antichymotrypsin) . It may react to reduced PSA at a higher concentration.

Product Specifications

CAS Number

9000-83-3

Specifications

Immunohistochemistry (FFPE) : 1:500-1:1000, Western blot (Non-reduced) : 1:1000-1:2500, ELISA: 1:100-1:5000

UniProt

P07288

Host

Rabbit

Reactivity

Human

Immunogen

Native PSA protein purified from human seminal fluid was used as the immunogen for the recombinant biotinylated Prostate Specific Antigen antibody.

Clonality

Recombinant Monoclonal

Isotype

IgG

Clone

RM323

Type

Recombinant

Applications

WB, IHC-P, ELISA

Purity

Protein A purified from animal origin-free supernatant

Format

Biotin Conjugate

Buffer

Antibody in PBS with 50% glycerol, 1% BSA and 0.09% sodium azide

Reconstitution

Store the recombinant biotinylated Prostate Specific Antigen antibody at -20oC.

Limitations

This recombinant biotinylated Prostate Specific Antigen antibody is available for research use only.

Storage Conditions

Store the recombinant biotinylated Prostate Specific Antigen antibody at -20°C.

Formulation

Antibody in PBS with 50% glycerol, 1% BSA and 0.09% sodium azide

Applications Notes

The stated application concentrations are suggested starting points. Titration of the recombinant biotinylated Prostate Specific Antigen antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Image Legend

A titer ELISA of PSA (purified from human seminal fluid) . The plate was coated with different amounts of PSA. A serial dilution of recombinant Prostate Specific Antigen antibody was used as the primary. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary.

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