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H2BK5ac Antibody / Acetyl-Histone H2B Lysine 5

This antibody reacts to Histone H2B acetylated at Lysine 5 (K5ac) . No cross reactivity with other acetylated Lysines in histones.

Product Specifications

CAS Number

9000-83-3

Specifications

Western blot: 0.01 µg/mL-1 µg/mL, ELISA: 0.2 µg/mL-1 µg/mL

UniProt

P33778

Host

Rabbit

Reactivity

Human

Immunogen

An acetyl-peptide corresponding to Acetyl-Histone H2B (Lys5) was used as the immunogen for the recombinant H2BK5ac antibody.

Clonality

Recombinant Monoclonal

Isotype

IgG

Clone

RM455

Type

Recombinant

Applications

WB, ELISA

Purity

Protein A purified from animal origin-free supernatant

Format

Purified

Buffer

1 mg/ml in PBS with 50% glycerol, 1% BSA and 0.09% sodium azide

Reconstitution

Store the recombinant H2BK5ac antibody at -20oC.

Limitations

This recombinant H2BK5ac antibody is available for research use only.

Storage Conditions

Store the recombinant H2BK5ac antibody at -20°C.

Formulation

1 mg/mL in PBS with 50% glycerol, 1% BSA and 0.09% sodium azide

Applications Notes

The stated application concentrations are suggested starting points. Titration of the recombinant H2BK5ac antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Image Legend

Western blot testing of acid extracts of HeLa cells, treated (+) or untreated (-) with sodium butyrate, using H2BK5ac antibody at 0.02 ug/ml.

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