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Phospho-Aurora A/B/C Antibody (pThr288/pThr232/pThr198)

This antibody reacts to human Aurora A/B/C only when phosphorylated at either Thr288, Thr232 or Thr198 respectively. There is no cross-reactivity to Aurora A/B/C that is not phosphorylated at these positions.

Product Specifications

CAS Number

9000-83-3

Specifications

Western blot: 1:1000-1:3000, Immunohistochemistry (FFPE) : 1:25-1:50

UniProt

Q9UQB9, Q96GD4, O14965

Host

Rabbit

Reactivity

Human

Immunogen

A phospho-peptide corresponding to Phospho-Aurora A (Thr288) /Aurora B (Thr232) /Aurora C (Thr198) was used as the immunogen for the phospho-Aurora A/B/C antibody.

Clonality

Recombinant Monoclonal

Isotype

IgG

Clone

RM454

Type

Recombinant

Applications

WB, IHC-P

Purity

Protein A purified from animal origin-free supernatant

Format

Purified

Buffer

Antibody in PBS with 50% glycerol, 1% BSA and 0.09% sodium azide

Reconstitution

Store the recombinant phospho-Aurora A/B/C antibody at -20oC.

Limitations

This recombinant phospho-Aurora A/B/C antibody is available for research use only.

Storage Conditions

Store the recombinant phospho-Aurora A/B/C antibody at -20°C.

Formulation

Antibody in PBS with 50% glycerol, 1% BSA and 0.09% sodium azide

Applications Notes

The stated application concentrations are suggested starting points. Titration of the recombinant phospho-Aurora A/B/C antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Image Legend

Western blot testing of lysate of human HeLa cells, non-treated (-) or treated (+) with Nocodazole and Calyculin A, using recombinant phospho-Aurora A/B/C antibody at a 1:2500 dilution.

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