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Phospho-EIF4E Antibody (pSer209)

This antibody reacts to human eIF4E only when phosphorylated at Ser209. There is no cross-reactivity to eIF4E that is not phosphorylated.

Product Specifications

CAS Number

9000-83-3

Specifications

Western blot: 1:500-1:1000

UniProt

P06730

Host

Rabbit

Reactivity

Human

Immunogen

The amino acid sequence surrounding pSer209 of human EIF4E protein was used as the immunogen for the phospho-EIF4E antibody.

Clonality

Recombinant Monoclonal

Isotype

IgG

Clone

RM452

Type

Recombinant

Applications

WB

Purity

Protein A purified from animal origin-free supernatant

Format

Purified

Buffer

Antibody in PBS with 50% glycerol, 1% BSA and 0.09% sodium azide

Reconstitution

Store the recombinant phospho-eIF4E antibody at -20oC.

Limitations

This recombinant phospho-EIF4E antibody is available for research use only.

Storage Conditions

Store the recombinant phospho-eIF4E antibody at -20°C.

Formulation

Antibody in PBS with 50% glycerol, 1% BSA and 0.09% sodium azide

Applications Notes

The stated application concentrations are suggested starting points. Titration of the recombinant phospho-EIF4E antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Image Legend

Western blot testing of lysate of human MCF-7 cells, non-treated (-) or treated (+) with fetal bovine serum, using recombinant phospho-EIF4E antibody at a 1:1000 dilution.

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