Detyrosinated Alpha Tubulin Antibody

This antibody reacts to detyrosinated alpha-Tubulin, a posttranslational modification of alpha-tubulin where the C-terminal tyrosine residue has been cleaved.

Product Specifications

CAS Number

9000-83-3

Specifications

Western blot: 1:500-1:1000, Immunocytochemistry/Immunofluorescence: 1:100-1:200

UniProt

Q71U36

Host

Rabbit

Reactivity

Human

Immunogen

A peptide corresponding to human dTyr alpha-tubulin (cleaved C-terminal tyrosine residue) was used as the immunogen for the Detyrosinated Alpha Tubulin antibody.

Clonality

Recombinant Monoclonal

Isotype

IgG

Clone

RM444

Type

Recombinant

Applications

WB, ICC

Purity

Protein A purified from animal origin-free supernatant

Format

Purified

Buffer

Antibody in PBS with 50% glycerol, 1% BSA and 0.09% sodium azide

Reconstitution

Store the recombinant Detyrosinated Alpha Tubulin antibody at -20oC.

Limitations

This recombinant Detyrosinated Alpha Tubulin antibody is available for research use only.

Storage Conditions

Store the recombinant Detyrosinated Alpha Tubulin antibody at -20°C.

Formulation

Antibody in PBS with 50% glycerol, 1% BSA and 0.09% sodium azide

Applications Notes

The stated application concentrations are suggested starting points. Titration of the recombinant Detyrosinated Alpha Tubulin antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Image Legend

Western blot analysis of human HeLa, U2OS, and CHL-1 cell lysates using Detyrosinated Alpha-Tubulin antibody at a 1:1000 dilution. Image courtesy of Dr. Takashi Hotta from Ohi Lab, University of Michigan, USA.