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H3K27cr Antibody / Crotonylated-Histone H3 Lysine 27

Selected Variant

R20417-100UG

Size: 100 µg

This antibody reacts to Histone H3 crotonylated at Lysine 27 (K27cr), and does not cross-react with acetylated or butyrylated Lysine 27. No cross reactivity with other crotonylated Lysines in histone H3.

Product Specifications

CAS Number

9000-83-3

Specifications

Western blot: 0.5 µg/mL-2 µg/mL, Dot blot: 0.5 µg/mL-2 µg/mL

UniProt

P84243

Host

Rabbit

Reactivity

Human

Immunogen

A crotonyl-peptide corresponding to Crotonyl-Histone H3 (Lys27) was used as the immunogen for the recombinant H3K27cr antibody.

Clonality

Recombinant Monoclonal

Isotype

IgG

Clone

RM401

Type

Recombinant

Applications

WB, Dot blot

Purity

Protein A purified from animal origin-free supernatant

Format

Purified

Buffer

1 mg/ml in PBS with 50% glycerol, 1% BSA and 0.09% sodium azide

Reconstitution

Store the recombinant H3K27cr antibody at -20oC.

Limitations

This recombinant H3K27cr antibody is available for research use only.

Storage Conditions

Store the recombinant H3K27cr antibody at -20°C.

Formulation

1 mg/mL in PBS with 50% glycerol, 1% BSA and 0.09% sodium azide

Applications Notes

The stated application concentrations are suggested starting points. Titration of the recombinant H3K27cr antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Image Legend

Western blot testing of acid extracts of HeLa cells with or without sodium crotonate treatment using recombinant H3K27cr at 0.5 ug/ml.

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