DDDDK Tag Antibody / FLAG Tag

This antibody reacts to recombinant proteins containing a DDDDK fused to either the amino or carboxy terminus. No cross reactivity with any endogenous protein.

Product Specifications

CAS Number

9000-83-3

Specifications

Western blot: 0.001 µg/mL-0.1 µg/mL

Host

Rabbit

Immunogen

Mixture of a peptide with DDDDK-Tag at the N-terminal and another peptide with DDDDK-Tag at the C-terminal was used as the immunogen for the DDDDK Tag antibody.

Clonality

Recombinant Monoclonal

Isotype

IgG

Clone

RM399

Type

Recombinant

Applications

Purity

Protein A purified from animal origin-free supernatant

Format

Purified

Buffer

1 mg/ml in PBS with 50% glycerol, 1% BSA and 0.09% sodium azide

Reconstitution

Store the recombinant DDDDK Tag antibody at -20oC.

Limitations

This recombinant DDDDK Tag antibody is available for research use only.

Storage Conditions

Store the recombinant DDDDK Tag antibody at -20°C.

Formulation

1 mg/mL in PBS with 50% glycerol, 1% BSA and 0.09% sodium azide

Applications Notes

The stated application concentrations are suggested starting points. Titration of the recombinant DDDDK Tag antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Image Legend

Western blot testing of 293T cells (1) transfected or (2) non-transfected with a DNA construct encoding DDDDK tag fusion protein, using recombinant DDDDK Tag antibody at 0.001ug/ml.