ALK Antibody / Anaplastic lymphoma kinase

This antibody reacts human total ALK protein including ALK fusion proteins.

Product Specifications

CAS Number

9000-83-3

Specifications

Immunohistochemistry (FFPE) : 1:500-1:1000, Western blot: 1:1000-1:2000

UniProt

Q9UM73

Host

Rabbit

Reactivity

Human

Immunogen

A peptide corresponding to the C-terminus of human ALK (Anaplastic lymphoma kinase) was used as the immunogen for the recombinant ALK antibody.

Clonality

Recombinant Monoclonal

Isotype

IgG

Clone

RM361

Type

Recombinant

Applications

WB, IHC-P

Purity

Protein A purified from animal origin-free supernatant

Format

Purified

Buffer

Antibody in PBS with 50% glycerol, 1% BSA and 0.09% sodium azide

Reconstitution

Store the recombinant ALK antibody at -20oC.

Limitations

This recombinant ALK antibody is available for research use only.

Storage Conditions

Store the recombinant ALK antibody at -20°C.

Formulation

Antibody in PBS with 50% glycerol, 1% BSA and 0.09% sodium azide

Applications Notes

The stated application concentrations are suggested starting points. Titration of the recombinant ALK antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Location

Cytoplasmic, nuclear

Image Legend

Western blot testing of the non-small cell lung cancer cell line H2228 cell lysate expressing EML4-ALK variant 3, using recombinant ALK antibody at 1:2000 dilution.