GLIS1 Antibody / GLI-similar 1

Glis1 (Glis Family Zinc Finger 1) is gene encoding a Kruppel-like protein of the same name whose locus is found on Chromosome 1p32.3. GLIS1 is a GLI (MIM 165220) -related Kruppel-like zinc finger protein that functions as an activator and repressor of transcription.

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 0.5-1 µg/mL, Immunohistochemistry (FFPE) : 2-5 µg/mL, Immunofluorescence: 5 µg/mL, Flow cytometry: 1-3ug/million cells, Direct ELISA: 0.1-0.5 µg/mL

UniProt

Q8NBF1

Host

Rabbit

Reactivity

Human

Immunogen

Recombinant human protein (amino acids M1-T620) was used as the immunogen for the GLIS1 antibody.

Clonality

Polyclonal

Isotype

IgG

Applications

WB, IHC-P, IF, FACS, Direct ELISA

Purity

Antigen affinity purified

Format

Antigen affinity purified

Buffer

Lyophilized from 1X PBS with 2% Trehalose

Reconstitution

After reconstitution, the GLIS1 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Limitations

This GLIS1 antibody is available for research use only.

Storage Conditions

After reconstitution, the GLIS1 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Formulation

0.5 mg/mL if reconstituted with 0.2ml sterile DI water

Applications Notes

Optimal dilution of the GLIS1 antibody should be determined by the researcher.

Location

Nuclear

Image Legend

IHC staining of FFPE human lung cancer tissue with GLIS1 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.