Malic enzyme 1 Antibody / ME1

NADP-dependent malic enzyme (Malic enzyme 1) is a protein that in humans is encoded by the ME1 gene. This gene encodes a cytosolic, NADP-dependent enzyme that generates NADPH for fatty acid biosynthesis. The activity of this enzyme, the reversible oxidative decarboxylation of malate, links the glycolytic and citric acid cycles. The regulation of expression for this gene is complex. Increased expression can result from elevated levels of thyroid hormones or by higher proportions of carbohydrates in the diet.

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 0.5-1 µg/mL, Immunohistochemistry (FFPE) : 2-5 µg/mL

UniProt

P48163

Host

Mouse

Reactivity

Human, Mouse, Rat

Immunogen

Recombinant human protein (amino acids M1-Q572) was used as the immunogen for the Malic enzyme 1 antibody.

Clonality

Monoclonal

Isotype

IgG1

Clone

5E5F7

Applications

WB, IHC-P

Purity

Antigen affinity purified

Format

Antigen affinity purified

Buffer

Lyophilized from 1X PBS with 2% Trehalose

Reconstitution

After reconstitution, the Malic enzyme 1 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Limitations

This Malic enzyme 1 antibody is available for research use only.

Storage Conditions

After reconstitution, the Malic enzyme 1 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Formulation

0.5 mg/mL if reconstituted with 0.2ml sterile DI water

Applications Notes

Optimal dilution of the Malic enzyme 1 antibody should be determined by the researcher.

Location

Cytoplasmic

Image Legend

IHC staining of FFPE human spleen tissue with Malic enzyme 1 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.