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RPL32 Antibody / 60S ribosomal protein L32

60S ribosomal protein L32 is a protein that in humans is encoded by the RPL32 gene. Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a ribosomal protein that is a component of the 60S subunit. The protein belongs to the L32E family of ribosomal proteins. It is located in the cytoplasm. Although some studies have mapped this gene to 3q13.3-q21, it is believed to map to 3p25-p24. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome. Alternatively spliced transcript variants encoding the same protein have been observed for this gene.

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 0.5-1 µg/mL, Immunohistochemistry (FFPE) : 2-5 µg/mL, Immunofluorescence: 5 µg/mL, Flow cytometry: 1-3ug/million cells

UniProt

P62910

Host

Rabbit

Reactivity

Human, Mouse, Rat

Immunogen

Amino acids KNRKAIVERAAQLAIR were used as the immunogen for the RPL32 antibody.

Clonality

Polyclonal

Isotype

IgG

Applications

WB, IHC-P, IF, FACS

Purity

Antigen affinity purified

Format

Antigen affinity purified

Buffer

Lyophilized from 1X PBS with 2% Trehalose

Reconstitution

After reconstitution, the RPL32 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Limitations

This RPL32 antibody is available for research use only.

Storage Conditions

After reconstitution, the RPL32 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Formulation

0.5 mg/mL if reconstituted with 0.2ml sterile DI water

Applications Notes

Optimal dilution of the RPL32 antibody should be determined by the researcher.

Location

Cytoplasmic

Image Legend

Western blot testing of 1) rat liver, 2) rat pancreas, 3) rat NRK, 4) mouse liver, 5) mouse pancreas, 6) rat stomach and 7) mouse HEPA1-6 cell lysate with RPL32 antibody. Predicted molecular weight ~18 kDa.
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