17HSD7 Antibody / HSD17B7

3-keto-steroid reductase is an enzyme that in humans is encoded by the HSD17B7 gene. HSD17B7 encodes an enzyme that functions both as a 17-beta-hydroxysteroid dehydrogenase (EC 1.1.1.62) in the biosynthesis of sex steroids and as a 3-ketosteroid reductase (EC 1.1.1.270) in the biosynthesis of cholesterol.

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 0.5-1 µg/mL, Immunohistochemistry (FFPE) : 2-5 µg/mL, Immunofluorescence: 5 µg/mL, Flow cytometry: 1-3ug/million cells, Direct ELISA: 0.1-0.5 µg/mL

UniProt

P56937

Host

Rabbit

Reactivity

Human, Rat

Immunogen

Recombinant human protein (amino acids L16-L336) was used as the immunogen for the 17HSD7 antibody.

Clonality

Polyclonal

Isotype

IgG

Applications

WB, IHC-P, IF, FACS, Direct ELISA

Purity

Antigen affinity purified

Format

Antigen affinity purified

Buffer

Lyophilized from 1X PBS with 2% Trehalose

Reconstitution

After reconstitution, the 17HSD7 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Limitations

This 17HSD7 antibody is available for research use only.

Storage Conditions

After reconstitution, the 17HSD7 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Formulation

0.5 mg/mL if reconstituted with 0.2ml sterile DI water

Applications Notes

Optimal dilution of the 17HSD7 antibody should be determined by the researcher.

Location

Cytoplasmic (ER)

Image Legend

IHC staining of FFPE human colorectal adenocarcinoma tissue with 17HSD7 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.