LIMA1 Antibody / EPLIN

LIM domain and actin-binding protein 1, also called EPLIN (Epithelial protein lost in neoplasm), is a protein that in humans is encoded by the LIMA1 gene. This gene encodes a cytoskeleton-associated protein that inhibits actin filament depolymerization and cross-links filaments in bundles. It is downregulated in some cancer cell lines. Alternatively spliced transcript variants encoding different isoforms have been described for this gene, and expression of some of the variants maybe independently regulated.

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 0.5-1 µg/mL, Immunohistochemistry (FFPE) : 2-5 µg/mL, Immunofluorescence: 5 µg/mL, Flow cytometry: 1-3ug/million cells, Direct ELISA: 0.1-0.5 µg/mL

UniProt

Q9UHB6

Host

Rabbit

Reactivity

Human

Immunogen

Recombinant human protein (amino acids R9-E744) was used as the immunogen for the LIMA1 antibody.

Clonality

Polyclonal

Isotype

IgG

Applications

WB, IHC-P, IF, FACS, Direct ELISA

Purity

Antigen affinity purified

Format

Antigen affinity purified

Buffer

Lyophilized from 1X PBS with 2% Trehalose

Reconstitution

After reconstitution, the LIMA1 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Limitations

This LIMA1 antibody is available for research use only.

Storage Conditions

After reconstitution, the LIMA1 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Formulation

0.5 mg/mL if reconstituted with 0.2ml sterile DI water

Applications Notes

Optimal dilution of the LIMA1 antibody should be determined by the researcher.

Location

Cell membrane, cytoplasm

Image Legend

IHC staining of FFPE human lung squamous cell carcinoma tissue with LIMA1 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.