CFIm68 Antibody / CPSF6

Cleavage and polyadenylation specificity factor subunit 6, also called Cleavage factor Im complex 68 kDa subunit (CFIm68), is a protein that in humans is encoded by the CPSF6 gene. The protein encoded by this gene is one subunit of a cleavage factor required for 3' RNA cleavage and polyadenylation processing. The interaction of the protein with the RNA is one of the earliest steps in the assembly of the 3' end processing complex and facilitates the recruitment of other processing factors. The cleavage factor complex is composed of four polypeptides. This gene encodes the 68kD subunit. It has a domain organization reminiscent of spliceosomal proteins.

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 0.5-1 µg/mL, Immunohistochemistry (FFPE) : 2-5 µg/mL, Immunofluorescence: 5 µg/mL, Flow cytometry: 1-3ug/million cells

UniProt

Q16630

Host

Mouse

Reactivity

Human, Mouse, Rat, Monkey

Immunogen

Recombinant human protein (amino acids R50-Q176) was used as the immunogen for the CFIm68 antibody.

Clonality

Monoclonal

Isotype

IgG1

Clone

3F11E1

Applications

WB, IHC-P, IF, FACS

Purity

Antigen affinity purified

Format

Antigen affinity purified

Buffer

Lyophilized from 1X PBS with 2% Trehalose

Reconstitution

After reconstitution, the CFIm68 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Limitations

This CFIm68 antibody is available for research use only.

Storage Conditions

After reconstitution, the CFIm68 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Formulation

0.5 mg/mL if reconstituted with 0.2ml sterile DI water

Applications Notes

Optimal dilution of the CFIm68 antibody should be determined by the researcher.

Location

Cytoplasmic, nuclear

Image Legend

IHC staining of FFPE human colorectal adenocarcinoma tissue with CFIm68 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.