Malate dehydrogenase 2 Antibody / MDH2

Malate dehydrogenase, mitochondrial, also known as malate dehydrogenase 2, is an enzyme that in humans is encoded by the MDH2 gene. Malate dehydrogenase catalyzes the reversible oxidation of malate to oxaloacetate, utilizing the NAD/NADH cofactor system in the citric acid cycle. The protein encoded by this gene is localized to the mitochondria and may play pivotal roles in the malate-aspartate shuttle that operates in the metabolic coordination between cytosol and mitochondria. Several transcript variants encoding different isoforms have been found for this gene.

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 0.5-1 µg/mL, Immunohistochemistry (FFPE) : 2-5 µg/mL, Immunofluorescence: 5 µg/mL

UniProt

P40926

Host

Mouse

Reactivity

Human

Immunogen

Recombinant human protein (amino acids A9-L223) was used as the immunogen for the Malate dehydrogenase 2 antibody.

Clonality

Monoclonal

Isotype

IgG2b

Clone

5D8C1

Applications

WB, IHC-P, IF

Purity

Antigen affinity purified

Format

Antigen affinity purified

Buffer

Lyophilized from 1X PBS with 2% Trehalose

Reconstitution

After reconstitution, the Malate dehydrogenase 2 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Limitations

This Malate dehydrogenase 2 antibody is available for research use only.

Storage Conditions

After reconstitution, the Malate dehydrogenase 2 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Formulation

0.5 mg/mL if reconstituted with 0.2ml sterile DI water

Applications Notes

Optimal dilution of the Malate dehydrogenase 2 antibody should be determined by the researcher.

Location

Cytoplasmic (mitochondria)

Image Legend

IHC staining of FFPE human cervical cancer tissue with Malate dehydrogenase 2 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.