HBEGF Antibody / DT-R

Heparin-binding EGF-like growth factor (HB-EGF), also called Diphtheria toxin receptor (DT-R), is a member of the EGF family of proteins that in humans is encoded by the HBEGF gene. Enables growth factor activity and heparin binding activity. Involved in several processes, including epidermal growth factor receptor signaling pathway; positive regulation of protein kinase B signaling; and positive regulation of wound healing. Located in cell surface and extracellular space. Implicated in glomerulosclerosis and perinatal necrotizing enterocolitis.

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 0.5-1 µg/mL, Immunohistochemistry (FFPE) : 2-5 µg/mL, Flow cytometry: 1-3ug/million cells, Immunofluorescence: 5 µg/mL

UniProt

Q99075

Host

Rabbit

Reactivity

Human, Mouse, Rat

Immunogen

Amino acids KKRDPCLRKYKDFCIHGECKYVKE were used as the immunogen for the HBEGF antibody.

Clonality

Polyclonal

Isotype

IgG

Applications

WB, IHC-P, FACS, IF

Purity

Antigen affinity purified

Format

Antigen affinity purified

Buffer

Lyophilized from 1X PBS with 2% Trehalose

Reconstitution

After reconstitution, the HBEGF antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Limitations

This HBEGF antibody is available for research use only.

Storage Conditions

After reconstitution, the HBEGF antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Formulation

0.5 mg/mL if reconstituted with 0.2ml sterile DI water

Applications Notes

Optimal dilution of the HBEGF antibody should be determined by the researcher.

Image Legend

Immunofluorescent staining of FFPE human colorectal adenocarcinoma tissue with HBEGF antibody (red) and DAPI nuclear stain (blue) . HIER: steam section in pH8 EDTA buffer for 20 min.