JUP Antibody / Junction Plakoglobin / Gamma Catenin

Junction plakoglobin (JUP), also known as gamma-catenin, is a protein that in humans is encoded by the JUP gene. It is a member of the catenin protein family and homologous to g-catenin, and it is mapped to 17q21.2. This gene encodes a major cytoplasmic protein that is the only known constituent common to submembranous plaques of both desmosomes and intermediate junctions. This protein forms distinct complexes with cadherins and desmosomal cadherins. Meanwhile, JUP may have distinct roles in Wnt signaling and cancer via differential effects on downstream target genes.

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 0.5-1 µg/mL, Immunohistochemistry (FFPE) : 2-5 µg/mL, Immunofluorescence: 5 µg/mL, Flow cytometry: 1-3ug/million cells

UniProt

P14923

Host

Mouse

Reactivity

Human, Mouse, Rat

Immunogen

Recombinant human protein (amino acids M556-A745) was used as the immunogen for the JUP antibody.

Clonality

Monoclonal

Isotype

IgG1

Clone

4C12D7

Applications

WB, IHC-P, IF, FACS

Purity

Antigen affinity purified

Format

Antigen affinity purified

Buffer

Lyophilized from 1X PBS with 2% Trehalose

Reconstitution

After reconstitution, the JUP antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Limitations

This JUP antibody is available for research use only.

Storage Conditions

After reconstitution, the JUP antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Formulation

0.5 mg/mL if reconstituted with 0.2ml sterile DI water

Applications Notes

Optimal dilution of the JUP antibody should be determined by the researcher.

Location

Cell surface, cytoplasmic, cell junctions

Image Legend

IHC staining of FFPE human lung adenocarcinoma tissue with JUP antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.