DUP Antibody / CDT1

CDT1 (Chromatin licensing and DNA replication factor 1), also called DUP (Double parked homolog) is a protein that in humans is encoded by the CDT1 gene. The protein encoded by this gene is involved in the formation of the pre-replication complex that is necessary for DNA replication. The encoded protein can bind geminin, which prevents replication and may function to prevent this protein from initiating replication at inappropriate origins. Phosphorylation of this protein by cyclin A-dependent kinases results in degradation of the protein.

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 0.5-1 µg/mL, Direct ELISA: 0.1-0.5 µg/mL

UniProt

Q9H211

Host

Rabbit

Reactivity

Human, Mouse

Immunogen

Recombinant human protein (amino acids R19-L357) was used as the immunogen for the DUP antibody.

Clonality

Polyclonal

Isotype

IgG

Applications

WB, Direct ELISA

Purity

Antigen affinity purified

Format

Antigen affinity purified

Buffer

Lyophilized from 1X PBS with 2% Trehalose

Reconstitution

After reconstitution, the DUP antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Limitations

This DUP antibody is available for research use only.

Storage Conditions

After reconstitution, the DUP antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Formulation

0.5 mg/mL if reconstituted with 0.2ml sterile DI water

Applications Notes

Optimal dilution of the DUP antibody should be determined by the researcher.

Image Legend

Western blot testing of 1) human MCF7, 2) human HeLa and 3) mouse brain tissue lysate with DUP antibody. Predicted molecular weight ~60 kDa, commonly observed at 60-70 kDa.