Epoxide hydrolase Antibody / EPHX1

Epoxide hydrolase 1 is an enzyme encoded by the EPHX1 gene in humans. Epoxide hydrolase is a critical biotransformation enzyme that converts epoxides from the degradation of aromatic compounds to trans-dihydrodiols which can be conjugated and excreted from the body. Epoxide hydrolase functions in both the activation and detoxification of epoxides. Mutations in this gene cause preeclampsia, epoxide hydrolase deficiency or increased epoxide hydrolase activity. Alternatively spliced transcript variants encoding the same protein have been found for this gene.

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 0.5-1 µg/mL, Flow cytometry: 1-3ug/million cells, Direct ELISA: 0.1-0.5 µg/mL

UniProt

P07099

Host

Rabbit

Reactivity

Human, Mouse, Rat

Immunogen

Recombinant human protein (amino acids A42-Q455) was used as the immunogen for the Epoxide hydrolase antibody.

Clonality

Polyclonal

Isotype

IgG

Applications

WB, FACS, Direct ELISA

Purity

Antigen affinity purified

Format

Antigen affinity purified

Buffer

Lyophilized from 1X PBS with 2% Trehalose

Reconstitution

After reconstitution, the Epoxide hydrolase antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Limitations

This Epoxide hydrolase antibody is available for research use only.

Storage Conditions

After reconstitution, the Epoxide hydrolase antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Formulation

0.5 mg/mL if reconstituted with 0.2ml sterile DI water

Applications Notes

Optimal dilution of the Epoxide hydrolase antibody should be determined by the researcher.

Image Legend

Flow cytometry testing of human 293T cells with Epoxide hydrolase antibody at 1ug/million cells (blocked with goat sera) ; Red=cells alone, Green=isotype control, Blue= Epoxide hydrolase antibody.