ACTA2 Antibody

Actin is a major component of the cytoskeleton and is present in most cell types.

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 0.5-1 µg/mL, Immunohistochemistry (FFPE) : 2-5 µg/mL, Immunohistochemistry (Frozen) : 2-5 µg/mL, Immunofluorescence: 5 µg/mL

UniProt

P62736

Host

Mouse

Reactivity

Human, Mouse, Rat

Immunogen

Amino acids 1-10 of alpha-smooth muscle actin was used as the immunogen for this ACTA2 antibody.

Clonality

Monoclonal

Isotype

IgG2a

Clone

1A4

Applications

WB, IHC-P, IHC-F, IF

Purity

Unpurified ascites

Format

Ascites

Buffer

Mouse ascites fluid with 2.5% BSA and 0.025% sodium azide

Reconstitution

After reconstitution, the ACTA2 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Limitations

This ACTA2 antibody is available for research use only.

Storage Conditions

After reconstitution, the ACTA2 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Formulation

0.5 mg/mL if reconstituted with 0.2ml sterile DI water

Applications Notes

The stated application concentrations are suggested starting points. Titration of the ACTA2 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Image Legend

Immunofluorescent staining of FFPE human placental tissue with ACTA2 antibody (green), CD31 antibody (red) and DAPI nuclear stain (blue) . HIER: steam section in pH8 EDTA buffer for 20 min.