ACTA2 Antibody
Actin is a major component of the cytoskeleton and is present in most cell types.
Product Specifications
CAS Number
9007-83-4
Specifications
Western blot: 0.5-1 µg/mL, Immunohistochemistry (FFPE) : 2-5 µg/mL, Immunohistochemistry (Frozen) : 2-5 µg/mL, Immunofluorescence: 5 µg/mL
UniProt
P62736
Host
Mouse
Reactivity
Human, Mouse, Rat
Immunogen
Amino acids 1-10 of alpha-smooth muscle actin was used as the immunogen for this ACTA2 antibody.
Clonality
Monoclonal
Isotype
IgG2a
Clone
1A4
Applications
WB, IHC-P, IHC-F, IF
Purity
Unpurified ascites
Format
Ascites
Buffer
Mouse ascites fluid with 2.5% BSA and 0.025% sodium azide
Reconstitution
After reconstitution, the ACTA2 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
Limitations
This ACTA2 antibody is available for research use only.
Storage Conditions
After reconstitution, the ACTA2 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.
Formulation
0.5 mg/mL if reconstituted with 0.2ml sterile DI water
Applications Notes
The stated application concentrations are suggested starting points. Titration of the ACTA2 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Image Legend
Immunofluorescent staining of FFPE human placental tissue with ACTA2 antibody (green), CD31 antibody (red) and DAPI nuclear stain (blue) . HIER: steam section in pH8 EDTA buffer for 20 min.
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