What is the catalog number for Immuno HRP-AEC, Anti-Chicken IgY (H+L)?

The catalog number for Immuno HRP-AEC, Anti-Chicken IgY (H+L) is IH-8043-50.

What sizes are available for Immuno HRP-AEC, Anti-Chicken IgY (H+L)?

Immuno HRP-AEC, Anti-Chicken IgY (H+L) is available in the following size(s): Each Reagent 50 mL.

Is Immuno HRP-AEC, Anti-Chicken IgY (H+L) in stock?

Immuno HRP-AEC, Anti-Chicken IgY (H+L) is currently made to order / not held in immediate stock. Contact us for lead time.

What is the recommended positive control for this assay?

A recommended positive control, where one is specified by the manufacturer, is listed on the datasheet or in the assay protocol.

How should this product be stored?

Storage conditions depend on the specific reagent and are listed on the product datasheet, with most antibodies and proteins requiring -20°C unless stated otherwise. It is your responsibility to store the product correctly immediately upon receipt; damage from improper storage after delivery is not eligible for replacement.

Is this product animal-origin-free?

Animal-origin-free status, where applicable, is noted on the datasheet under formulation details.

Immuno HRP-AEC, Anti-Chicken IgY (H+L)

Immunohistochemistry (IHC)/Immunocytochemistry (ICC) is the localization of antigens by the use of antigens in tissue sections/cells by the use of labeled antibodies as specific reagents through antigen-antibody interactions that are visualized by a marker such as fluorescent dye, enzyme, radioactive element or colloidal gold. Several IHC techniques are commonly used: labeled biotin secondary antibody streptavidin-peroxidase (LBSASP), HRP anti-HRP, ABC, catalyzed signal amplification, polymer system and others, to detect antigens on tissue and cell. In this kit the first layer is unlabeled primary antibody, the second layer is biotinylated secondary antibody, the third layer is Enzyme-Streptavidin conjugate (HRP-Streptavidin) to replace the complex of avidin-biotin peroxidase. The enzyme is then visualized by application of the substrate chromogen solution to produce different colorimetric end products.

Product Specifications

CAS Number

9007-83-4

Applications

IHC, ICC

Assay Principle

Mixing of Chromogen reagent 6: 1. To 5 ml of distilled or deionized water in a test tube, add two drop of reagent B buffer, mix well. 2. Add one drop of reagent C chromogen, mix well. 3. Add one drop of reagent S substrate, mix well. This ready to use reagent is good for several hours. IHC/ICC procedure for frozen sections, paraffin sections and cell smears. 1. Deparafinize and hydrate tissue sections through xylene or other clearing agents and graded alcohols.(For frozen sections or cell smears; use unfixed, acetone fixed or appropriate fixative for the antigen in question; for cell smears it may be necessary to permealize the cell by detergent, please refer to antibody protocol) 2. Wash 2-3 with distilled or deionized water. 3. Incubate sections/cell smear in Endoblocker (#1) for 5-10 minutes at room temperature or 37°C, wash with distilled water. 4. Note: If antigen retriever (Trypsin AR-6541, Pronase AR-6542, Pepsin AR-6543, Citrate buffer AR-6544, Buffer w EDTA pH 8.5 AR-6545, Tris buffer pH 10 AR-6546) is required it can be applied at this step. Please refer to data sheet for the primary antibody. 5. Wash slide with PBS or Tris saline (with 0.02-0.05% nonionic detergent, Triton X100, Tween 20 or NP-40) or washing buffer (Immuno Automation buffer IBSC cat # AR-6561) 3-5X. 6. Incubate sections/ cell smear in Protein blocking solution (#2) for 10 minutes. at RT or 37ºC 7. Wash slide with PBS 1X, this step can be omitted.. 8. Incubate sections/cell smear in primary antibody (NOT SUPPLIED, ONLY BUFFER IS SUPPLIED FOR DILUTION) for 20-30 minutes at room temperature or 37°C. (For more information, refer to instructions for primary antibody) 9. Wash slide with PBS 5-7X 10. Incubate with biotinylated secondary antibody (#4) for 15 minutes at room temp. or 37°C. 11. Wash slide 5-7 times with buffer. Caution: Peroxidase reagents are destroyed by sodium azide and should be avoided in all buffers and regents. 12. Incubate with Streptavidin-Peroxidase reagent (5) for 10 minutes at room temperature or 37ºC. 13. Wash slide with PBS for 5-7 X. 14. Wash slide with deionized or distilled for 2-3X. 15. Incubate with AEC reagent (#6) for 5-10 minutes at room temperature or 37ºC. 16. Wash slide with distilled or deionized water 5-7X. 17. Incubate with hematoxylin counterstain (#7) 30-60 seconds. 18. Wash slide with tap water, distilled water, followed by PBS buffer. 19. Keep in this buffer for 2-3 minutes till hematoxylin change color from purple to blue. 20. Wash slide with distilled or deionized water. Now this slide is ready to be mounted with aqueous mounting medium, ImmunoHistoMount (#8) 21. Please see instructions for ImmunoHistoMount(The data sheet is provided)