Trimethoprim ELISA Kit

Trimethoprim (TMP) belongs to the list of essential medicines of World Health Organization. It is an antibiotic used mainly in the treatment of bacterial infections of human bladder, urinary tract and middle ear. TMP may be used against any susceptible gram-negative, aerobic bacterial species including E. coli, Enterobacter, Klebsiella, Providencia, Morganella, Citrobacter, Salmonella and Shigella. It may be also used in combination with other drugs to prevent kidney infections, lung infections and certain types of chronic inflammatory or autoimmune diseases. TMP is a potent dihydrofolate reductase (DHFR) inhibitor. It binds to dihydrofolate reductase and blocks the thymidine synthesis pathway thereby interfering with the bacterial DNA synthesis. Even though TMP is a very safe drug, it has some side effects such as nausea, diarrhea, rash, itching etc. BioVision’s Trimethoprim ELISA Kit is a competitive enzyme immunoassay to determine the TMP concentration in various biological samples such as serum and urine. In this assay, the TMP antigen is pre-coated on the 96-well plate. TMP in the sample and the TMP antigen on the plate compete for binding to the anti-TMP antibody. After the addition of the HRP conjugate that binds to the captured TMP antibody, TMB substrate is added and a color signal is developed, which is measured at 450 nm. The OD value of the sample is inversely proportional to the TMP concentration in the sample. The OD value is then compared to the TMP standard curve and the TMP concentration is obtained. The kit is simple, easy and reliable tool to determine the TMP concentration (1 - 82 ng/ml) in approximately 90 minutes. The limit of detection of the kit is 0.5 ng/ml.