Fluorescein Conjugated Affinity Purified anti-Rat IgG [H&L] [Goat]
Selected Variant
GTR18290235
Size: 250 µg
Minimum Cross Reactivity to Bovine, Chicken, Goat, Guinea Pig, Hamster, Horse, Human, Mouse, Rabbit and Sheep Serum Proteins
Product Specifications
Reactivity
Rat
Conjugation
Unconjugated
Field of Research
Cell Biology
Purification
This product was prepared from monospecific antiserum by immunoaffinity chromatography using Rat IgG coupled to agarose beads followed by solid phase adsorption (s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Fluorescein, anti-Goat Serum, Rat IgG, Rat IgG F (ab) 2 F (c) and Rat Serum. No reaction was observed against Rat IgG F (ab) 2 F (c) or Bovine, Chicken, Goat, Guinea Pig, Hamster, Horse, Human, Mouse, Rabbit and Sheep Serum Proteins. Specificity was confirmed by ELISA at less than 1% cross reactivity against other mouse or human heavy or light chain isotypes.
Concentration
1.0 mg/ml (by UV absorbance at 280 nm)
Purity
This product was prepared from monospecific antiserum by immunoaffinity chromatography using Rat IgG coupled to agarose beads followed by solid phase adsorption (s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Fluorescein, anti-Goat Serum, Rat IgG, Rat IgG F (ab) 2 F (c) and Rat Serum. No reaction was observed against Rat IgG F (ab) 2 F (c) or Bovine, Chicken, Goat, Guinea Pig, Hamster, Horse, Human, Mouse, Rabbit and Sheep Serum Proteins. Specificity was confirmed by ELISA at less than 1% cross reactivity against other mouse or human heavy or light chain isotypes.
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Notes
For research use only.
Applications Notes
Not specified. Optimal titers for applications should be determined by the researcher.
Tested Applications
FACS
Host or Source
Goat
Preservative
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Price
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