Alkaline Phosphatase Conjugated Affinity Purified anti-Cat IgG [H&L] [Rabbit]
Alkaline Phosphatase Conjugated Affinity Purified anti-Cat IgG [H&L] [Rabbit]
Product Specifications
Reactivity
Feline
Immunogen
Cat IgG whole molecule
Conjugation
Unconjugated
Field of Research
Metabolism Research
Purification
This product was prepared from monospecific antiserum by immunoaffinity chromatography using Cat IgG coupled to agarose beads. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Alkaline Phosphatase (calf intestine), anti-Rabbit Serum, Cat IgG and Cat Serum.
Concentration
1.0 mg/ml (by UV absorbance at 280 nm)
Purity
This product was prepared from monospecific antiserum by immunoaffinity chromatography using Cat IgG coupled to agarose beads. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Alkaline Phosphatase (calf intestine), anti-Rabbit Serum, Cat IgG and Cat Serum.
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Notes
For research use only.
Applications Notes
This product has been assayed against 1.0 μg of Cat IgG in a standard capture ELISA using pNPP p-nitrophenyl phosphate code # NPP-10 as a substrate for 30 minutes at room temperature. A working dilution of 1:4,000 to 1:18,000 is suggested for this product. Optimal titers for other applications should be determined by the researcher.
Tested Applications
DOT, ELISA, IHC, WB
Host or Source
Rabbit
Preservative
0.05M Tris Chloride, 0.15M Sodium Chloride, 0.001M Magnesium Chloride, 0.0001M Zinc Chloride, 50% (v/v) Glycerol; pH 8.0
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