Alkaline Phosphatase Conjugated Affinity Purified anti-Dog IgG [H&L] [Rabbit]
Selected Variant
GTR18289657
Size: 250 µg
Alkaline Phosphatase Conjugated Affinity Purified anti-Dog IgG [H&L] [Rabbit]
Product Specifications
Reactivity
Canine
Immunogen
Dog IgG whole molecule
Conjugation
Unconjugated
Field of Research
Signal Transduction
Purification
This product was prepared from monospecific antiserum by immunoaffinity chromatography using Dog IgG coupled to agarose beads followed by solid phase adsorption (s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Alkaline Phosphatase (calf intestine), anti-Rabbit Serum, Dog IgG and Dog Serum.
Concentration
1.0 mg/ml (by UV absorbance at 280 nm)
Purity
This product was prepared from monospecific antiserum by immunoaffinity chromatography using Dog IgG coupled to agarose beads followed by solid phase adsorption (s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Alkaline Phosphatase (calf intestine), anti-Rabbit Serum, Dog IgG and Dog Serum.
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Notes
For research use only.
Applications Notes
This product has been assayed against 1.0 μg of Dog IgG in a standard capture ELISA using pNPP p-nitrophenyl phosphate code # NPP-10 as a substrate for 30 minutes at room temperature. A working dilution of 1:1,000 to 1:5,000 is suggested for this product. Optimal titers for other applications should be determined by the researcher.
Tested Applications
DOT, ELISA, IHC, WB
Host or Source
Rabbit
Preservative
0.05M Tris Chloride, 0.15M Sodium Chloride, 0.001M Magnesium Chloride, 0.0001M Zinc Chloride, 50% (v/v) Glycerol; pH 8.0
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