Alkaline Phosphatase Conjugated Affinity Purified anti-Donkey IgG [H&L] [Goat]
Selected Variant
GTR18289546
Size: 250 µg
Alkaline Phosphatase Conjugated Affinity Purified anti-Donkey IgG [H&L] [Goat]
Product Specifications
Reactivity
Donkey
Immunogen
Donkey IgG whole molecule
Conjugation
Unconjugated
Field of Research
Cell Biology
Purification
This product was prepared from monospecific antiserum by immunoaffinity chromatography using Donkey IgG coupled to agarose beads. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Alkaline Phosphatase (calf intestine), anti-Goat Serum, Donkey IgG, and Donkey Serum.
Concentration
0.59 mg/ml (by UV absorbance at 280 nm)
Purity
This product was prepared from monospecific antiserum by immunoaffinity chromatography using Donkey IgG coupled to agarose beads. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Alkaline Phosphatase (calf intestine), anti-Goat Serum, Donkey IgG, and Donkey Serum.
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Notes
For research use only.
Applications Notes
This product has been assayed against 1.0 μg of Donkey IgG in a standard capture ELISA using pNPP p-nitrophenyl phosphate code # NPP-10 as a substrate for 30 minutes at room temperature. A working dilution of 1:2,000 to 1:16,000 of the reconstitution concentration is suggested for this product. Optimal titers for other applications should be determined by the researcher.
Tested Applications
DOT, ELISA, IHC, WB
Host or Source
Goat
Preservative
0.05M Tris Chloride, 0.15M Sodium Chloride, 0.001M Magnesium Chloride, 0.0001M Zinc Chloride, 50% (v/v) Glycerol; pH 8.0
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