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Anti-HUMAN IgA (alpha chain) (GOAT) Peroxidase Conjugated

Anti-HUMAN IgA (alpha chain) (GOAT) Peroxidase Conjugated

Product Specifications

Reactivity

Human

Immunogen

Human IgA a alpha chain

Conjugation

Unconjugated

Field of Research

Cell Biology

Purification

This product was prepared from monospecific antiserum by immunoaffinity chromatography using Human IgA coupled to agarose beads followed by solid phase adsorption (s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Goat Serum, Human IgA and Human Serum. Specificity was confirmed by ELISA at less than 1% cross reactivity against other Human heavy or light chain isotypes.

Concentration

1.0 mg/ml (by UV absorbance at 280 nm)

Purity

This product was prepared from monospecific antiserum by immunoaffinity chromatography using Human IgA coupled to agarose beads followed by solid phase adsorption (s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Goat Serum, Human IgA and Human Serum. Specificity was confirmed by ELISA at less than 1% cross reactivity against other Human heavy or light chain isotypes.

Storage Conditions

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Notes

For research use only.

Applications Notes

ELISA: 1:10,000 - 1:150,000 / WB: 1:1,000 - 1:5,000 / IHC: 1:500 - 1:2,500 / OTHER: User Optimized

Tested Applications

ELISA, IHC, WB

Host or Source

Goat

Preservative

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

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