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Peroxidase Conjugated Affinity Purified anti-Chicken IgG [H&L] [Rabbit]

Peroxidase Conjugated Affinity Purified anti-Chicken IgG [H&L] [Rabbit]

Product Specifications

Reactivity

Gallus

Immunogen

Chicken IgG whole molecule

Conjugation

Unconjugated

Purification

This product was prepared from monospecific antiserum by immunoaffinity chromatography using Chicken IgG coupled to agarose beads followed by solid phase adsorption (s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti- Peroxidase, anti-Rabbit Serum, Chicken IgG and Chicken Serum.

Concentration

1.5 mg/ml (by UV absorbance at 280 nm)

Purity

This product was prepared from monospecific antiserum by immunoaffinity chromatography using Chicken IgG coupled to agarose beads followed by solid phase adsorption (s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti- Peroxidase, anti-Rabbit Serum, Chicken IgG and Chicken Serum.

Storage Conditions

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Notes

For research use only.

Applications Notes

This product has been assayed against 1.0 μg of Chicken IgG in a standard capture ELISA using ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature. A working dilution of 1:4,000 to 1:16,000 is suggested for this product. Optimal titers for other applications should be determined by the researcher.

Tested Applications

DOT, ELISA, WB

Host or Source

Rabbit

Preservative

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

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