Peroxidase Conjugated Affinity Purified anti-Chicken IgG [H&L] [Rabbit]
Peroxidase Conjugated Affinity Purified anti-Chicken IgG [H&L] [Rabbit]
Product Specifications
Reactivity
Gallus
Immunogen
Chicken IgG whole molecule
Conjugation
Unconjugated
Purification
This product was prepared from monospecific antiserum by immunoaffinity chromatography using Chicken IgG coupled to agarose beads followed by solid phase adsorption (s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti- Peroxidase, anti-Rabbit Serum, Chicken IgG and Chicken Serum.
Concentration
1.5 mg/ml (by UV absorbance at 280 nm)
Purity
This product was prepared from monospecific antiserum by immunoaffinity chromatography using Chicken IgG coupled to agarose beads followed by solid phase adsorption (s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti- Peroxidase, anti-Rabbit Serum, Chicken IgG and Chicken Serum.
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Notes
For research use only.
Applications Notes
This product has been assayed against 1.0 μg of Chicken IgG in a standard capture ELISA using ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature. A working dilution of 1:4,000 to 1:16,000 is suggested for this product. Optimal titers for other applications should be determined by the researcher.
Tested Applications
DOT, ELISA, WB
Host or Source
Rabbit
Preservative
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
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