Peroxidase Conjugated Affinity Purified anti-Golden Syrian Hamster IgG [H&L] [Goat]

This product was prepared from monospecific antiserum by immunoaffinity chromatography using Golden Syrian Hamster IgG coupled to agarose beads followed by solid phase adsorption (s) to remove any unwanted reactivities and extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, anti-Peroxidase, Golden Syrian Hamster IgG and Golden Syrian Hamster Serum. No reaction was observed against Bovine, Chicken, Goat, Guinea Pig, Horse, Human, Mouse, Rabbit, Rat and Sheep Serum Proteins. Greatly reduced reactivity will occur against Armenian Hamster IgG.

This product was prepared from monospecific antiserum by immunoaffinity chromatography using Golden Syrian Hamster IgG coupled to agarose beads followed by solid phase adsorption (s) to remove any unwanted reactivities and extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, anti-Peroxidase, Golden Syrian Hamster IgG and Golden Syrian Hamster Serum. No reaction was observed against Bovine, Chicken, Goat, Guinea Pig, Horse, Human, Mouse, Rabbit, Rat and Sheep Serum Proteins. Greatly reduced reactivity will occur against Armenian Hamster IgG.

This product has been assayed against 1.0 μg of Hamster IgG in a standard capture ELISA using ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature. A working dilution of 1:25,000 to 1:125,000 is suggested for this product. Optimal titers for other applications should be determined by the researcher.