Peroxidase Conjugated Affinity Purified anti-Hamster IgG [H&L] [Rabbit]
Peroxidase Conjugated Affinity Purified anti-Hamster IgG [H&L] [Rabbit]
Product Specifications
Reactivity
Hamster
Immunogen
Hamster IgG whole molecule
Conjugation
Unconjugated
Purification
This product was prepared from monospecific antiserum by immunoaffinity chromatography using Hamster IgG coupled to agarose. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Rabbit Serum, Hamster IgG and Hamster Serum.
Concentration
1.5 mg/ml (by UV absorbance at 280 nm)
Purity
This product was prepared from monospecific antiserum by immunoaffinity chromatography using Hamster IgG coupled to agarose. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Rabbit Serum, Hamster IgG and Hamster Serum.
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Notes
For research use only.
Applications Notes
This product has been assayed against 1.0 μg of Hamster IgG in a standard capture ELISA using ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature. A working dilution of 1:4,000 to 1:20,000 of the reconstitution concentration is suggested for this product. Optimal titers for other applications should be determined by the researcher.
Tested Applications
DOT, ELISA, WB
Host or Source
Rabbit
Preservative
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
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