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Peroxidase Conjugated Affinity Purified anti-Hamster IgG [H&L] [Rabbit]

Selected Variant

GTR18289504

Size: 250 µg

Peroxidase Conjugated Affinity Purified anti-Hamster IgG [H&L] [Rabbit]

Product Specifications

Reactivity

Hamster

Immunogen

Hamster IgG whole molecule

Conjugation

Unconjugated

Purification

This product was prepared from monospecific antiserum by immunoaffinity chromatography using Hamster IgG coupled to agarose. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Rabbit Serum, Hamster IgG and Hamster Serum.

Concentration

1.5 mg/ml (by UV absorbance at 280 nm)

Purity

This product was prepared from monospecific antiserum by immunoaffinity chromatography using Hamster IgG coupled to agarose. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Rabbit Serum, Hamster IgG and Hamster Serum.

Storage Conditions

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Notes

For research use only.

Applications Notes

This product has been assayed against 1.0 μg of Hamster IgG in a standard capture ELISA using ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature. A working dilution of 1:4,000 to 1:20,000 of the reconstitution concentration is suggested for this product. Optimal titers for other applications should be determined by the researcher.

Tested Applications

DOT, ELISA, WB

Host or Source

Rabbit

Preservative

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

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