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Peroxidase Conjugated Affinity Purified anti-Horse IgG [H&L] [Rabbit]

Peroxidase Conjugated Affinity Purified anti-Horse IgG [H&L] [Rabbit]

Product Specifications

Reactivity

Equine

Immunogen

Horse IgG whole molecule

Conjugation

Unconjugated

Purification

This product was prepared from monospecific antiserum by immunoaffinity chromatography using Horse IgG coupled to agarose beads. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Rabbit Serum, Horse IgG and Horse Serum.

Concentration

2.0 mg/ml (by UV absorbance at 280 nm)

Purity

This product was prepared from monospecific antiserum by immunoaffinity chromatography using Horse IgG coupled to agarose beads. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Rabbit Serum, Horse IgG and Horse Serum.

Storage Conditions

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Notes

For research use only.

Applications Notes

This product has been assayed against 1.0 μg of Horse IgG in a standard capture ELISA using ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature. A working dilution of 1:3,000 to 1:20,000 of the reconstitution concentration is suggested for this product. Optimal titers for other applications should be determined by the researcher.

Tested Applications

DOT, ELISA, WB

Host or Source

Rabbit

Preservative

0.01 M Sodium Phosphate, 0.14 M Sodium Chloride, pH 7.4

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