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Peroxidase Conjugated Affinity Purified anti-Human IgG [H&L] [Rabbit]

Minimum Cross reactivity to Mouse Serum Proteins

Product Specifications

Reactivity

Human

Immunogen

Human IgG whole molecule

Conjugation

Unconjugated

Purification

This product was prepared from monospecific antiserum by immunoaffinity chromatography using Human IgG coupled to agarose beads followed by solid phase adsorption (s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Rabbit Serum, Human IgG and Human Serum. No reaction was observed against Mouse Serum Proteins.

Concentration

2.0 mg/ml (by UV absorbance at 280 nm)

Purity

This product was prepared from monospecific antiserum by immunoaffinity chromatography using Human IgG coupled to agarose beads followed by solid phase adsorption (s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Rabbit Serum, Human IgG and Human Serum. No reaction was observed against Mouse Serum Proteins.

Storage Conditions

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Notes

For research use only.

Applications Notes

ELISA 1:180,000 WESTERN BLOT 1:2,000 - 1:10,000 IMMUNOHISTOCHEMISTRY 1:1,000 - 1:5,000 OTHER APPLICATIONS User Optimized

Tested Applications

DOT, ELISA, WB

Host or Source

Rabbit

Preservative

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

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