Peroxidase Conjugated Affinity Purified anti-Human IgG [H&L] [Rabbit]
Minimum Cross reactivity to Mouse Serum Proteins
Product Specifications
Reactivity
Human
Immunogen
Human IgG whole molecule
Conjugation
Unconjugated
Purification
This product was prepared from monospecific antiserum by immunoaffinity chromatography using Human IgG coupled to agarose beads followed by solid phase adsorption (s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Rabbit Serum, Human IgG and Human Serum. No reaction was observed against Mouse Serum Proteins.
Concentration
2.0 mg/ml (by UV absorbance at 280 nm)
Purity
This product was prepared from monospecific antiserum by immunoaffinity chromatography using Human IgG coupled to agarose beads followed by solid phase adsorption (s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Rabbit Serum, Human IgG and Human Serum. No reaction was observed against Mouse Serum Proteins.
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Notes
For research use only.
Applications Notes
ELISA 1:180,000 WESTERN BLOT 1:2,000 - 1:10,000 IMMUNOHISTOCHEMISTRY 1:1,000 - 1:5,000 OTHER APPLICATIONS User Optimized
Tested Applications
DOT, ELISA, WB
Host or Source
Rabbit
Preservative
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
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