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Phycoerythrin Conjugated Affinity Purified anti-Rat IgG [H&L] [Goat]

Minimum Cross Reactivity to Bovine, Chicken, Goat, Guinea Pig, Horse, Human, Mouse, Rabbit and Sheep Serum Proteins

Product Specifications

Reactivity

Rat

Immunogen

Rat IgG whole molecule

Conjugation

Unconjugated

Field of Research

Cell Biology

Purification

This product was prepared from monospecific antiserum by immunoaffinity chromatography using Rat IgG coupled to agarose beads followed by solid phase adsorption (s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Phycoerythrin, anti-Goat Serum, Rat IgG and Rat Serum. No reaction was observed against Bovine, Chicken, Goat, Guinea Pig, Horse, Human, Mouse, Rabbit and Sheep Serum Proteins.

Concentration

1.0 mg/ml (by UV absorbance at 280 nm) . OD566nm/OD280nm ratio: 2.0

Purity

This product was prepared from monospecific antiserum by immunoaffinity chromatography using Rat IgG coupled to agarose beads followed by solid phase adsorption (s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Phycoerythrin, anti-Goat Serum, Rat IgG and Rat Serum. No reaction was observed against Bovine, Chicken, Goat, Guinea Pig, Horse, Human, Mouse, Rabbit and Sheep Serum Proteins.

Storage Conditions

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Notes

For research use only.

Applications Notes

The maximum amount of reagent required to stain 1 x 106 cells in flow cytometry is approximately 1.0 μg of conjugate. Lesser amounts of reagent may be sufficient for staining. Optimal titers for other applications should be determined by the researcher. As a general guideline dilutions of 1:100 to 1:250 should be suitable for most applications.

Tested Applications

FACS

Host or Source

Goat

Preservative

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

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