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IL2RA Antibody (Biotin)

IL2RA Antibody (Biotin)

Product Specifications

Product Name Alternative

Soluble IL-2 receptor, TAC-antigen, CD25 antigen

UniProt

P01589

Reactivity

Human

Immunogen

Produced from sera of rabbits immunized with highly pure Recombinant Human sIL-2 Receptor alpha. Anti-Human sIL-2 Receptor alpha-specific antibody was purified by affinity chromatography and then biotinylated.

Target

IL2RA

Clonality

Polyclonal

Conjugation

Biotin

Field of Research

Immunology & Inflammation

Purification

SIL-2 Receptor alpha-specific antibody was purified by affinity chromatography and then biotinylated

Concentration

Batch dependent

Form

Lyophilized

Storage Conditions

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Notes

For research use only.

Applications Notes

Sandwich ELISA: To detect Human sIL-2 Receptor alpha by sandwich ELISA (using 100μl/well) a concentration of 0.25-1.0 μg/ml of this antibody is required. This biotinylated polyclonal antibody, in conjunction with Biorbyt’s Polyclonal Anti-Human sIL-2 Receptor alpha as a capture antibody, allows the detection of at least 2000-4000 pg/ml of Recombinant Human sIL-2 Receptor alpha. Western Blot To detect Human sIL-2 Receptor alpha by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 μg/ml. When used in conjunction with compatible development reagents the detection limit for Recombinant Human sIL-2 Receptor alpha is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.

Tested Applications

ELISA, WB

NCBI Accession Number

P01589

Host or Source

Rabbit

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