Il3 Antibody (Biotin)

Product Specifications

Product Name Alternative

MCGF (Mast cell growth factor), Multi-CSF, HCGF, P-cell stimulation factor

UniProt

P01586

Reactivity

Mouse

Immunogen

Produced from sera of rabbits immunized with highly pure Recombinant Murine IL-3. Anti-Murine IL-3-specific antibody was purified by affinity chromatography and then biotinylated.

Target

Il3

Clonality

Polyclonal

Conjugation

Biotin

Field of Research

Immunology & Inflammation

Purification

IL-3-specific antibody was purified by affinity chromatography and then biotinylated

Concentration

Batch dependent

Form

Lyophilized

Storage Conditions

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Notes

For research use only.

Applications Notes

Sandwich ELISA: To detect mIL-3 by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.25 – 1.0 μg/ml of this antibody is required. This biotinylated polyclonal antibody, in conjunction with Biorbyt’s Polyclonal Anti-Murine IL-3 as a capture antibody, allows the detection of at least 0.2 – 0.4 ng/well of recombinant mIL-3. Western Blot To detect mIL-3 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant mIL-3 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.