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Il13 Antibody (Biotin)

Il13 Antibody (Biotin)

Product Specifications

Product Name Alternative

NC300 (Human), P600 (Murine)

UniProt

P42203

Reactivity

Rat

Immunogen

Produced from sera of rabbits immunized with highly pure recombinant Rat IL-13. Anti-Rat IL-13-specific antibody was purified by affinity chromatography and then biotinylated.

Target

Il13

Clonality

Polyclonal

Conjugation

Biotin

Field of Research

Immunology & Inflammation

Purification

IL-13 specific antibody was purified by affinity chromatography and then biotinylated

Concentration

Batch dependent

Form

Lyophilized

Storage Conditions

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Notes

For research use only.

Applications Notes

Sandwich ELISA: To detect Rat IL-13 by sandwich ELISA (using 100 ml/well) a concentration of 0.25 – 1.0 μg/ml of this antibody is required. This biotinylated polyclonal antibody, in conjunction with Biorbyt’s Polyclonal Anti- Rat IL-13 as a capture antibody, allows the detection of at least 0.2 – 0.4 ng/well of recombinant Rat IL-13. Western Blot To detect Rat IL-13 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 mg/ml. When used in conjunction with compatible development reagents the detection limit for recombinant Rat IL-13 is 1.5 – 3.0 ng/lane, under either reducing or non-reducing conditions.

Tested Applications

ELISA, WB

NCBI Accession Number

P42203

Host or Source

Rabbit

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