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Il7 Antibody (Biotin)

Il7 Antibody (Biotin)

Product Specifications

Product Name Alternative

Lymphopoietin 1 (LP-1), pre-B cell factor

UniProt

P56478

Reactivity

Rat

Immunogen

Produced from sera of rabbits immunized with highly pure recombinant Rat IL-7. Anti-Rat IL-7 specific antibody was purified by affinity chromatography and then biotinylated.

Target

Il7

Clonality

Polyclonal

Conjugation

Biotin

Field of Research

Immunology & Inflammation

Purification

IL-7 specific antibody was purified by affinity chromatography and then biotinylated

Concentration

Batch dependent

Form

Lyophilized

Storage Conditions

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Notes

For research use only.

Applications Notes

Sandwich ELISA: To detect Rat IL-7 by sandwich ELISA (using 100 ml/well) a concentration of 0.25 – 1.0 μg/ml of this antibody is required. This biotinylated polyclonal antibody, in conjunction with Biorbyt’s Polyclonal Anti-Rat IL-7 as a capture antibody, allows the detection of at least 0.2 – 0.4 ng/well of recombinant Rat IL-7. Western Blot To detect Rat IL-7 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 mg/ml. When used in conjunction with compatible development reagents the detection limit for recombinant Rat IL-7 is 1.5 – 3.0 ng/lane, under either reducing or non-reducing conditions.

Tested Applications

ELISA, WB

NCBI Accession Number

P56478

Host or Source

Rabbit

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