SPP1 Antibody (Biotin)
SPP1 Antibody (Biotin)
Product Specifications
Product Name Alternative
Bone sialoprotein 1, Secreted phosphoprotein 1, SPP-1, Nephropontin, Uropontin
UniProt
P10451
Reactivity
Human
Immunogen
Produced from sera of rabbits immunized with highly pure recombinant Human Osteopontin. Anti-Human Osteopontin-specific antibody was purified by affinity chromatography and then biotinylated.
Target
SPP1
Clonality
Polyclonal
Conjugation
Biotin
Field of Research
Cancer Biology
Purification
Osteopontin-specific antibody was purified by affinity chromatography and then biotinylated
Concentration
Batch dependent
Form
Lyophilized
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Notes
For research use only.
Applications Notes
Sandwich ELISA: To detect Human Osteopontin by sandwich ELISA (using 100 ml/well), a concentration of 0.25 – 1.0 μg/ml of this antibody is required. This biotinylated polyclonal antibody, in conjunction with Biorbyt’s Polyclonal Anti- Human Osteopontin as a capture antibody, allows the detection of at least 0.2 – 0.4 ng/well of recombinant Human Osteopontin. Western Blot To detect Human Osteopontin by Western Blot analysis, this antibody can be used at a concentration of 0.1 – 0.2 mg/ml. When used in conjunction with compatible development reagents, the detection limit for recombinant Human Osteopontin is 1.5 – 3.0 ng/lane, under either reducing or non-reducing conditions.
Tested Applications
ELISA, WB
NCBI Accession Number
P10451
Host or Source
Rabbit
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