EPO Antibody (Biotin)
EPO Antibody (Biotin)
Product Specifications
Product Name Alternative
Erythropoietin, Epoetin
UniProt
P01588
Reactivity
Human
Immunogen
Produced from sera of rabbits immunized with highly pure Recombinant Human EPO. Anti-Human EPO-specific antibody was purified by affinity chromatography and then biotinylated.
Target
EPO
Clonality
Polyclonal
Conjugation
Biotin
Field of Research
Cell Biology, Immunology & Inflammation, Neuroscience
Purification
EPO-specific antibody was purified by affinity chromatography and then biotinylated
Concentration
Batch dependent
Form
Lyophilized
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Notes
For research use only.
Applications Notes
Sandwich ELISA: To detect Human EPO by sandwich ELISA (using 100μl/well), a concentration of 0.25-1.0 μg/ml of this antibody is required. This biotinylated polyclonal antibody, in conjunction with Biorbyt’s Polyclonal Anti-Human EPO as a capture antibody, allows the detection of at least 2000-4000 pg/ml of Recombinant Human EPO. Western Blot To detect Human EPO by Western Blot analysis, this antibody can be used at a concentration of 0.1-0.2 μg/ml. When used in conjunction with compatible development reagents, the detection limit for Recombinant Human EPO is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
Tested Applications
ELISA, WB
NCBI Accession Number
P01588
Host or Source
Rabbit
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