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EPO Antibody (Biotin)

EPO Antibody (Biotin)

Product Specifications

Product Name Alternative

Erythropoietin, Epoetin

UniProt

P01588

Reactivity

Human

Immunogen

Produced from sera of rabbits immunized with highly pure Recombinant Human EPO. Anti-Human EPO-specific antibody was purified by affinity chromatography and then biotinylated.

Target

EPO

Clonality

Polyclonal

Conjugation

Biotin

Field of Research

Cell Biology, Immunology & Inflammation, Neuroscience

Purification

EPO-specific antibody was purified by affinity chromatography and then biotinylated

Concentration

Batch dependent

Form

Lyophilized

Storage Conditions

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Notes

For research use only.

Applications Notes

Sandwich ELISA: To detect Human EPO by sandwich ELISA (using 100μl/well), a concentration of 0.25-1.0 μg/ml of this antibody is required. This biotinylated polyclonal antibody, in conjunction with Biorbyt’s Polyclonal Anti-Human EPO as a capture antibody, allows the detection of at least 2000-4000 pg/ml of Recombinant Human EPO. Western Blot To detect Human EPO by Western Blot analysis, this antibody can be used at a concentration of 0.1-0.2 μg/ml. When used in conjunction with compatible development reagents, the detection limit for Recombinant Human EPO is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.

Tested Applications

ELISA, WB

NCBI Accession Number

P01588

Host or Source

Rabbit

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